Nanopore sequencing is a third generation sequencing technology used for direct sequencing of polynucleotides in the form of a DNA or RNA molecule.
Nanopore sequencing allows a single molecule of DNA or RNA to be sequenced without the need for PCR amplification or chemical labeling of the sample. It can sequence long reads up to 2Mb.
Nanopore sequencing has the potential to offer relatively low-cost genotyping and rapid processing of samples with the ability to display results in real-time.
Applications include rapid identification of viral pathogens, monitoring epidemics, environmental monitoring, food safety monitoring, human genome sequencing, plant genome sequencing, monitoring of antibiotic resistance, haplotyping and others.